Journal: Journal of Veterinary Internal Medicine

Article Title: Investigation of the presence of specific neural antibodies in dogs with epilepsy or dyskinesia using murine and human assays

doi: 10.1111/jvim.16744

Figure Lengend Snippet: Canine anti‐IgLON5 antibodies. Immunofluorescence studies: Diluted serum (1:20) from a dog from the epilepsy/dyskinesia group (Golden Retriever, female, 3 years) is incubated with IgLON5‐transfected human embryonic kidney (HEK) cells. Binding of antibodies to cell surfaces is visualized by two different secondary anti‐canine antibodies coupled with immunofluorescence dyes and subsequently overlaid. The results demonstrate that there are canine anti‐IgLON5 IgG antibodies. Endpoint titration gave a titer of 1:40 (not shown). (A) anti‐canine IgG heavy and light chain (a sensitive secondary antibody), coupled with the red Alexa 594 dye. (B) overlay; yellow: double‐stainings. (C) anti‐canine IgG Fc fragment (the more specific secondary antibody), coupled with the green Alexa 488 dye. Bar: 25 μm.

Article Snippet: Secondary AB was a polyclonal rabbit anti‐dog immunoglobulin‐G (IgG) AB directed against canine IgG heavy and light chains (catalog no. 304‐065‐003; Jackson ImmunoResearch/Dianova) conjugated with red Alexa Fluor 594 used at a dilution of 1:100 and incubated for 30 minutes at room temperature; nuclei were counterstained with Hoechst 33342, 1:10 000.

Techniques: Immunofluorescence, Incubation, Transfection, Binding Assay, Titration

Journal: The Journal of veterinary medical science

Article Title: Effects of salivary gland extract from Rhipicephalus sanguineus on IgG subclass production and cytokine mRNA expression in mononuclear cells of canine peripheral blood.

doi: 10.1292/jvms.65.137

Figure Lengend Snippet: Fig. 1. Effects of salivary gland extract (SGE) from partially engorged females of Rhipicephalus sanguineus on IgG1 and IgG2 production by the mononuclear cells from canine periph- eral blood, stimulated with concanavalin A (ConA). Cells (5 × 105/ml) were cultured with an optimal concentration of ConA (1 µg/ml) and various concentrations of SGE or PBS as controls. Asterisks (*) show significant differences (P<0.05) from the control. Short vertical bars represent standard errors. n=6.

Article Snippet: The horseradish peroxidaseconjugated goat anti-dog IgG1 (1:20,000 in PBS containing 0.05% Tween 20 (PBS-T), IgG1-heavy chain, BETHYL, U.S.A.) and the sheep anti-dog IgG2 (1:10,000 in PBS-T, IgG2-heavy chain, BETHYL, U.S.A.) were used to detect the reaction.

Techniques: Cell Culture, Concentration Assay, Control